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anti-5 bromodeoxyuridine (BrdU) antibody
概述
| 抗原: | 5 bromodeoxyuridine (BrdU) |
| 克隆: | 单克隆抗体 (MOBU-1) |
| 应用范围: | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC) |
| 目标物种: | All Species |
| 抗体来源: | Mouse |
|
8 references available |
| 规格: | |
| 价格: | 301,50 $ (加上运费$25和增值税, Invoice in EUR) |
| 货号: | ABIN93883 |
| 发货时间: | Ships within 1 to 3 Business days |
详细信息 » anti-5 bromodeoxyuridine (BrdU) antibody
| 抗原 | 5 bromodeoxyuridine (BrdU) |
| 免疫原 | 5-bromodeoxyuridine conjugated with hemocyanine. |
| 目标物种 | All Species |
| 克隆 | 单克隆抗体 |
| 状态 | Purified |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
| 克隆 | MOBU-1 |
| 抗体来源 | Mouse |
| 特异性 | The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). |
使用细节 » anti-5 bromodeoxyuridine (BrdU) antibody
| 应用范围 | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC) |
| 应用备注 | Immunocytochemistry: 2 µg/ml. Works very well for Immunohistochemistry with paraffin embedded sections. |
| 浓度 | 1 mg/ml |
| 纯度 | > 95% (by SDS-PAGE) |
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 缓冲液 | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| 贮藏 | Store at 2-8 |
| 研究领域 | Cell Cycle |
| 限制 | For Research Use only |
参考文献 » anti-5 bromodeoxyuridine (BrdU) antibody
| 参考文献 |
Schielen, Van Der Pijl, Bleumink et al.: "Local popliteal lymph node reactions to hexachlorobenzene and pentachlorobenzene: comparison with systemic effects." In: Immunopharmacology
, Vol. 31, Issue 02. Mrz, pp. 171-81, 1997 (PubMed)
Peters, Ganesh, Kuipers et al.: "Epithelial cell proliferative activity of Barrett's esophagus: methodology and correlation with traditional cancer risk markers." In: Digestive diseases and sciences , Vol. 43, Issue 7, pp. 1501-6, 1998 (PubMed) Buckiovua, Kubuinovua, Soukup et al.: "Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects." In: The International journal of developmental biology , Vol. 42, Issue 5, pp. 737-40, 1998 (PubMed) Stanek, Kiss, Raska: "Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription." In: European journal of cell biology , Vol. 79, Issue 3, pp. 202-7, 2000 (PubMed) Peters, Ganesh, Kuipers et al.: "Effect of elimination of acid reflux on epithelial cell proliferative activity of Barrett esophagus." In: Scandinavian journal of gastroenterology , Vol. 35, Issue 12, pp. 1238-44, 2001 (PubMed) Swee, Bosco, Malissen et al.: "Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment." In: Blood , Vol. 113, Issue 25, pp. 6277-87, 2009 (PubMed) Gamper, Koh, Ruau et al.: "GAR22: a novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells." In: Experimental hematology , Vol. 37, Issue 5, pp. 539-548.e4, 2009 (PubMed) Johnson, Phua, Bennett et al.: "Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells." In: Nucleic acids research , Vol. 37, Issue 14, pp. e98, 2009 (PubMed) |
图像 » anti-5 bromodeoxyuridine (BrdU) antibody
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