产品细节
anti-Thymine Dimer antibody (HRP)
概述
| 抗原: | Thymine Dimer |
| 克隆: | 单克隆抗体 (KTM53) |
| 应用范围: | In situ hybridization (ISH) |
| 目标物种: | Human, Monkey |
| 抗体来源: | Mouse |
| 偶联物: | HRP |
| 规格: | |
| 价格: | 460,00 $ (加上运费$25和增值税, Invoice in EUR) |
| 货号: | ABIN187268 |
| 发货时间: | Ships within 7 to 10 Business Days |
详细信息 » anti-Thymine Dimer antibody (HRP)
| 抗原 | Thymine Dimer |
| 免疫原 | UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes. |
| 目标物种 | Human, Monkey |
| 克隆 | 单克隆抗体 |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4. |
| 克隆 | KTM53 |
| 抗体来源 | Mouse |
| 特异性 | Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products. |
使用细节 » anti-Thymine Dimer antibody (HRP)
| 应用范围 | In situ hybridization (ISH) |
| 应用备注 | In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher. |
| 偶联物 | HRP |
| 缓冲液 | 100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration. |
| 贮藏 | Store at -20 °C. Aliquot to avoid freeze/thaw cycles. |
| 研究领域 | DNA/RNA, Cancer |
| 限制 | For Research Use only |

