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anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody
概述
| 抗原: | ATP-binding cassette, subfamily B, member 1 (ABCB1) |
| 克隆: | 单克隆抗体 (MRK16) |
| 应用范围: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF) |
| 目标物种: | Human |
| 抗体来源: | Mouse |
|
2 references available |
| 规格: | |
| 价格: | 5.599,57 ¥ (加上运费和增值税, Invoice in EUR) |
| 货号: | ABIN187222 |
| 发货时间: | Ships within 7 to 10 Business Days |
详细信息 » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody
| 抗原 | ATP-binding cassette, subfamily B, member 1 (ABCB1) |
| 基因ID | 5243 |
| 目标物种 | Human |
| 克隆 | 单克隆抗体 |
| 状态 | Purified |
| 抗体同种型 | IgG2a »相配的二抗 |
| 描述 | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] Also known as: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, ABCB1. |
| 克隆 | MRK16 |
| 抗体来源 | Mouse |
使用细节 » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody
| 应用范围 | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF) |
| 应用备注 | Potential Uses: cancer multidrug resistance studies, anti-cancer agent screening, AIDS multi-drug resistance studies, nervous system research, immunology research and transplant rejection studies, endocrine/steroid research, endothelium cell and brain P-glycoprotein research. Flow cytometry: For 5 x 105 pelleted cells use 50 µL of 10-100 µg/mL. Immunoprecipitation: Generally use between 25 µg/mL and 50 µg/mL. Immunohistochemistry: Generally use between 0.1 µg/mL and 10 µg/mL. Appropriate dilution must be decided upon according to staining method, conditions, etc. Frozen tissue staining can be done provided that mild fixation treatment is used, such as 4% paraformaldehyde. Acetone, paraffin, methyl alcohol, and ethyl alcohol should not be used as a fixative. Iodine labeling can also be performed on MRK16. Western blot: Does not react. Apparently, the epitope recognized by this antibody is lost in the presence of protein denaturing agents such as SDS. The optimal dilution for a specific application should be determined by the researcher. |
| 缓冲液 | 300 µL of 500 µg/mL monoclonal antibody in PBS, with protein stabilizer, 0.1% sodium azide. Purified by Protein A affinity chromatography. Page 1 of 2 |
| 贮藏 | Store at -20 °C. Avoid repeated freeze/thaw cycles. |
| 限制 | For Research Use only |
参考文献 » anti-ATP-binding cassette, subfamily B, member 1 (ABCB1) (Extracellular Epitope) antibody
| 参考文献 |
de Figueiredo-Pontes, Pintao, Oliveira et al.: "Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia." In: Cytometry. Part B, Clinical cytometry
, Vol. 74, Issue 3, pp. 163-8, 2008 (PubMed)
Valera, de Freitas Cortez, de Paula Queiroz et al.: "Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine." In: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery , Vol. 25, Issue 1, pp. 39-45, 2008 (PubMed) |
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