产品细节
anti-Transglutaminase II antibody
概述
| 抗原: | Transglutaminase II |
| 克隆: | 单克隆抗体 (CUB 7402) |
| 应用范围: | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| 目标物种: | Human, Dog (Canine), Rabbit, Guinea Pig |
| 抗体来源: | Mouse |
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1 reference available |
| 规格: | |
| 价格: | 493,00 $ (加上运费$25和增值税, Invoice in EUR) |
| 货号: | ABIN107743 |
| 发货时间: | Ships within 5 Business days |
详细信息 » anti-Transglutaminase II antibody
| 抗原 | Transglutaminase II |
| 免疫原 | BALB/C mice were injected with purified guinea pig liver TGase II. |
| 目标物种 | Human, Dog (Canine), Rabbit, Guinea Pig |
| 克隆 | 单克隆抗体 |
| 状态 | Ig Fraction |
| 抗体同种型 | IgG1 »相配的二抗 |
| 克隆 | CUB 7402 |
| 抗体来源 | Mouse |
| 特异性 | This antibody reacts with a 77-85 kD protein known as Transglutaminase II (TGase II). TGase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. Different tissue and cell types express varying amounts of tissue Transglutaminase. This antibody cross-reacts with dog, rabbit and Guinea pig. |
使用细节 » anti-Transglutaminase II antibody
| 应用范围 | Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| 应用备注 | Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix |
| 浓度 | 100ug/ml approximately |
| 缓冲液 | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| 贮藏 | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| 研究领域 | Enzymes, Extracellular Matrix |
| 限制 | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
参考文献 » anti-Transglutaminase II antibody
| 参考文献 |
Birckbichler, Upchurch, Patterson et al.: "A monoclonal antibody to cellular transglutaminase." In: Hybridoma
, Vol. 4, Issue 2, pp. 179-86, 1985 (PubMed)
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